Invitrogen™ Phospho-IKK alpha/beta (Ser176, Ser180) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5114586

Product Code. 17217353

Description

Antibody detects endogenous levels of IKK alpha/ beta only when phosphorylated at Ser176/180.

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

Specifications

• Antigen: Phospho-IKK alpha/beta (Ser176, Ser180)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CHUK
• Gene Alias: AI132552; AI256658; AIM-1; CHUK; Chuk1; component of inhibitor of nuclear factor kappa B kinase complex; conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; Fbx24; Fbxo24; I kappa B kinase 1; i kappa-B kinase alpha; IkappaB kinase; I-kappa-B kinase 1; I-kappa-B kinase 2; IkappaB kinase alpha; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkB kinase alpha subunit; IkB kinase-alpha; IkB kinase-beta; ikBKA; Ikbkb; IKK alpha; IKK[b]; IKK1; IKK2; IKK-2; IKKA; IKK-A; IKK-a kinase; IKK-alpha; Ikkb; IKK-B; IKKbeta; IKK-beta; IMD15; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; inhibitor of nuclear factor kappa B kinase subunit beta; inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NFkappaB inhibitor kinase alpha; nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; TCF16; TCF-16; TCF17; Transcription factor 16
• Host Species: Rabbit
• Purification Method: Sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1147, 12675, 16150, 309361, 3551, 84351
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide
• Gene Accession No.: O14920, O15111, O88351, Q60680, Q9QY78
• Gene Symbols: CHUK, Ikbkb
• Immunogen: A synthesized peptide derived from human IKK alpha/ beta around the phosphorylation site of Ser176/180.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG