Invitrogen™ Phospho-GRF-1 (Tyr1087) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA540199

Product Code. 15506891

Description

This antibody is predicted to react with mouse and rat based on sequence homology.

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

Specifications

• Antigen: Phospho-GRF-1 (Tyr1087)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARHGAP35
• Gene Alias: 6430596G11Rik; AI841135; ARHGAP35; GAP-associated protein p190; glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF1; GRF-1; GRLF1; KIAA1722; LOW QUALITY PROTEIN: rho GTPase-activating protein 35; MGC10745; mKIAA1722; P190 RhoGAP; P190A; p190-A; p190ARHOGAP; p190RhoGAP; rho GAP p190A; Rho GTPase activating protein 35; rho GTPase-activating protein 35; Rho-GAP protein; p190-A; GRLF1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 232906, 2909, 306400
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.5% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P81128, Q91YM2, Q9NRY4
• Gene Symbols: ARHGAP35
• Immunogen: Synthesized peptide derived from human GRF-1 around the phosphorylation site of Y1087.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG