Invitrogen™ Phospho-DARPP-32 (Thr75) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA14611

Product Code. 11528940

Description

This antibody is predicted to react with bovine, canine, chicken, human, mouse and non-human primate based on 100% sequence homology. In Western blot, this antibody detects an ~32 kDa protein representing the Phospho-Thr75 DARPP-32 protein.

DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac™.

Specifications

• Antigen: Phospho-DARPP-32 (Thr75)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ppp1r1b
• Gene Alias: AU040756; DARPP32; DARPP-32; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; Ppp1r1b; PPR1B; protein pho; protein phosphatase 1 regulatory inhibitor subunit 1B; protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19049, 360616
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.25 mg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: Q60829, Q6J4I0
• Gene Symbols: Ppp1r1b
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat PPP1R1B conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG