Invitrogen™ Phospho-c-Jun (Ser63) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA517890

Product Code. 11597130

Description

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. It is not recommended to aliquot this antibody. This antibody does not recognize the phosphorylated forms of JunD or JunB.

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

Specifications

• Antigen: Phospho-c-Jun (Ser63)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: JUN
• Gene Alias: Activator protein 1; AH119; AP1; AP-1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; c-Jun; c-jun transcription factor; enhancer-binding protein AP1; I79_009953; immediate early; JUN; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; Junc; JUND; p39; proto-oncogene c-Jun; Rjg-9; transcription factor; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16476, 24516, 3725, 396913
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 153 μg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P05412, P05627, P17325, P56432
• Gene Symbols: JUN
• Immunogen: Synthetic phosphopeptide corresponding to residues around pSer63 of human c-Jun.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Pig
• Product Type: Antibody
• Isotype: IgG