Invitrogen™ Phospho-Bora (Ser497) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA564657

Product Code. 15712726

Description

Phospho-Bora (Ser497) Polyclonal Antibody detects endogenous levels of Bora only when phosphorylated at Ser497.

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Specifications

• Antigen: Phospho-Bora (Ser497)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BORA
• Gene Alias: 6720463M24Rik; AI317232; aurora borealis; BORA; bora, aurora kinase A activator; C13orf34; HsBora; Protein aurora borealis; RGD1309522; RP11-342J4.2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 306102, 79866
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q5M864, Q6PGQ7
• Gene Symbols: BORA
• Immunogen: A synthesized peptide derived from human BORA (Phospho-Ser497).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG