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Invitrogen™ AKT (Phospho) [pS473] Multispecies InstantOne™ ELISA Kit

InstantOne ELISA

Brand:  Invitrogen™ 85-86042-11

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540777

  • £372.00 / 96 tests


Estimated Shipment: 02-11-2021
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Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate seal

Description

Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Specifications

Specifications

P31749
ELISA Kit
ELISA
Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Cell Lysate,20 μL
1 hr. 20 min.
Human
96 Tests
Cell Lysates
Colorimetric Microplate Reader
HRP
Biotin
207
RUO
2°C to 8°C
1 hr. 10 min.
PKB, Protein Kinase B, RAC-PK-alpha
Human
AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2
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