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Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-9107-42
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46. 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3. In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation. Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation. Studies have identified several kinases that phosphorylate 4E-BP1. For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin. Applications Reported: This V3NTY24 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This V3NTY24 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Phospho-4EBP1 (Thr36, Thr45) Monoclonal antibody specifically detects Phospho-4EBP1 (Thr36, Thr45) in Human, Mouse samples. It is validated for Flow Cytometry.Specifications
Phospho-4EBP1 (Thr36, Thr45) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Q13541, Q60876 | |
EIF4EBP1 | |
Affinity chromatography | |
RUO | |
13685, 1978 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
V3NTY24 | |
eFluor 660 | |
EIF4EBP1 | |
4EBP1; 4E-BP1; AA959816; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1 | |
Mouse | |
100 Tests | |
Primary | |
Human, Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.