Invitrogen™ PHLPP2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA534435

Product Code. 15375361

Description

A suggested positive control is SW480 cell lysate. PA5-34435 can be used with blocking peptide PEP-1477.

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). [UniProt].

Specifications

• Antigen: PHLPP2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Phlpp2
• Gene Alias: AI481772; C130044A18Rik; GTPase activating protein and VPS9 domains 1; KIAA0931; PH domain and leucine rich repeat protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPP2; Phlppl; PHLPP-like; PPM3B; protein phosphatase, Mg2+/Mn2+ dependent 3B; RGD1562857
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23035
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q6ZVD8
• Gene Symbols: Phlpp2
• Immunogen: A 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG