Thermo Scientific™ Phire™ Hot Start II DNA Polymerase

Optimize routine and high throughput PCR applications requiring speed and specifity with this novel hot start DNA polymerase.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: F-122L

PHIRE HOT START II 1000U

UNSPSC: 41106300

Code: 87

Additional Details:
Additional Details: Weight: 1.71460kg



Product Code. 10247467

Quantity Price
1 £ 414.0 / 1000 reactions
In Stock 1
EU Stock 65
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Description and Specification

Specification

Description Phire™ Hot Start II DNA Polymerase
For Use With (Application) Fast PCR, routine PCR, high-throughput PCR, long-range PCR, DHPLC, microarray
Includes Phire Hot Start II DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer provides 1.5 mM MgCl2 in the final 1x concentration.
No. of Reactions 1000
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.

Phire Hot Start II DNA Polymerase is an upgraded version of the original Phire Hot Start DNA Polymerase. Due to improved Affibody-based hot start technology the polymerase has no annealing temperature limitations and it is extremely fast (extension time of only 10-15 s/kb). The unique dsDNA-binding domain makes Phire Hot Start II DNA Polymerase very robust and capable of amplifying long DNA fragments with high yields. The Affibody-based inactivation method of Phire Hot Start DNA Polymerase increases the specificity of the amplification at ambient temperatures but allows immediate and complete reactivation of the enzyme at standard cycling temperatures.
  • Quick hot start: No reactivation step
  • Fast enzyme: Amplify four to five times faster than with hot start Taq
  • Robust: Minimal reaction optimization due to high inhibitor tolerance
  • High yields: Abundant products due to high efficiency
  • Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq
  • No annealing temperature limitations

Includes:

Phire Hot Start II DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer provides 1.5 mM MgCl2 in the final 1x concentration.

Recommended for:

Fast PCR, routine PCR, high-throughput PCR, long-range PCR, DHPLC, microarray

Documentation

Technical documents