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Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure
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Nucleic acid extraction and purification.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Specifications
Specifications
| Chemical Name or Material | Phenol:Chloroform:Isoamyl Alcohol (25:24:1) |
| UN Number | UN2922 |
| Grade | Molecular Biology |
| Packaging | Plastic bottle |
| Quantity | 400 mL |
| Physical Form | Liquid |
Frequently Asked Questions (FAQs)
Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure should be stored at 4 degrees C.
We recommend the following Phenol/Chloroform RNA Isolation Method:
A. Guanidine HCI Homogenization Buffer for RNA Isolation: 8.0M Guanidine HCI, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample. Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Cat. No. J75829).
C. RNA Resuspension Buffer: TE, pH 7.5
Phenol:Chloroform:lsoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer. When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:lsoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.
Safety and Handling
- 400ML Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure
- Danger
- Acute toxicity Category 3
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 1
- Germ cell mutagenicity Category 2
- Reproductive toxicity Category 2
- Skin corrosion/irritation Category 1 B
- Specific target organ toxicity Category 1
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H318-Causes serious eye damage.
- H331-Toxic if inhaled.
- H341-Suspected of causing genetic defects.
- H351-Suspected of causing cancer.
- H361d-Suspected of damaging the unborn child.
- H372-Causes damage to organs.
- EUH066-Repeated exposure may cause skin dryness or cracking.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- MIXTURE LIST-Contains : phenol, chloroform,isoamyl alcohol
RUO – Research Use Only
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