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  5. Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure

Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure

Product Code. 15865408 Shop All Thermo Scientific Products
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Quantity:
100 mL
400 mL
Unit Size:
100mL
400mL
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Product Code. 15865408

Brand: Thermo Scientific J75831.AE

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Description

Nucleic acid extraction and purification

Nucleic acid extraction and purification.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Phenol:Chloroform:Isoamyl Alcohol (25:24:1)
UN Number UN2922
Grade Molecular Biology
Packaging Plastic bottle
Quantity 100 mL
Physical Form Liquid

Frequently Asked Questions (FAQs)

How should I store Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN)?

Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure should be stored at 4 degrees C.
Do you have instructions for RNA isolation with Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN)?

We recommend the following Phenol/Chloroform RNA Isolation Method:
A. Guanidine HCI Homogenization Buffer for RNA Isolation: 8.0M Guanidine HCI, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample. Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Cat. No. J75829).
C. RNA Resuspension Buffer: TE, pH 7.5
I received my order of Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN) and the pH is 6.7, but the product page says the pH should be 8.0. Why is that?

Phenol:Chloroform:lsoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer. When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:lsoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.

RUO – Research Use Only

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