Invitrogen™ PHAX Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA532138

Product Code. 13466707

Description

Recommended positive controls: 293T. Predicted reactivity: Mouse (94%), Rat (92%), Chicken (82%), Rhesus Monkey (98%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Specifications

• Antigen: PHAX
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PHAX
• Gene Alias: 26 kDa resiniferatoxin-binding protein; 2810055C14Rik; 4933427L19Rik; AU018701; AU018854; cytosolic resiniferatoxin-binding protein; D18Ertd65e; p55; PHAX; Phosphorylated adapter RNA export protein; phosphorylated adaptor for RNA export; phosphorylation regulated; RBP-2; RBP-26; resiniferatoxin-binding protein 2; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export adapter (phosphorylation regulated); RNA U, small nuclear RNA export adaptor; RNA U, small nuclear RNA export adaptor (phosphorylation regulated); Rnuxa; RTX-42
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51808
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9H814
• Gene Symbols: PHAX
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PHAX. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG