Invitrogen™ PFKL Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA521685

Product Code. 13249058

Description

Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (95%), Rat (95%), Chicken (88%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

Specifications

• Antigen: PFKL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PFKL
• Gene Alias: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; AA407869; ATP-dependent 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255; ATP-PFK; ATP-PFK {ECO:0000255; EC 2.7.1.11; HAMAP-Rule:MF_03184}; K6PL; liver-type 1-phosphofructokinase; Pfkb; PFK-B; Pfkl; Pfk-l; Phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver; phosphofructokinase, liver type; phosphofructokinase, liver, B-type; phosphohexokinase; phosphohexokinase {ECO:0000255
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18641, 5211
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1.23 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P12382, P17858
• Gene Symbols: PFKL
• Immunogen: Recombinant fragment corresponding to a region within amino acids 428 and 674 of PFKL (Uniprot ID#P17858).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG