missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Periostin Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHPOSTN

 View more versions of this product

Product Code. 15415113

  • £538.00 / 1 set
Estimated Shipment: 05-11-2024
to see stock.
Explore more special offers
Add to basket
This item is not returnable. View return policy

Description

Description

The Human Periostin ELISA quantitates Hu Periostin in human serum,plasma,or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PN. Principle of the method The Human Periostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Q15063
80 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
10631
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Extracellular Matrix & Cell Adhesion
2°C to 8°C
Human
4 hr. 45 min.
0.082-60 ng/mL
80pg/mL to 60ng/mL
Biotin
Cell Structure & Organelle Proteins
This ELISA pair antibody detects human Periostin. Other species not determined.
Human
Colorimetric Microplate Reader
OSF-2,OSF2,PDLPOSTN,PN
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
RP11-412K4.1, OSF-2, OSF2, PDLPOSTN, PN, periostin
1 hr. 20 min.
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers