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Perforin, clone: dG9 (delta G9), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9994-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218327

  • £55.57 / 25µg


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Description

Description

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Fluorochrome conjugated dG9 (delta G9) is recommended for use in intracellular flow cytometry.).Applications Tested: This dG9 (delta G9) antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Perforin
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P14222
cytolysin, FLH2, HPLH2, lymphocyte pore forming protein, lymphocyte pore-forming protein, perforin 1 (pore forming protein), perforin-1, PFN1, PFP, RP11-710A11.3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Immunoprecipitation
dG9 (delta G9)
Unconjugated
PRF1
Purified
PRF1
IgG2b, kappa
25μg
Store at 2-8°C.
Primary
5551
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