Eurofins DiscoverX PDGFR α (550-end, V561D) Protein, Active

Product Code. 17350634

Brand: Eurofins DiscoverX 14735M

Description

Active human, N-terminal 6His-tagged, recombinant, human PDGFR alpha, amino acids 550 - end, containing the V561D mutation. This V561D mutation has been found in some patients with gastrointestinal stromal tumors and confers constitutive activity.

Receptors that are tyrosine protein kinases include those receptors for platelet-derived growth factor (PDGF), insulin, epidermal growth factor (EGF) and certain lymphokines. Receptor tyrosine kinases are cell surface transmembrane proteins responsible for intracellular signal transduction. They are expressed in several cell types and, after activation by growth factor binding, trigger a series of intracellular pathways leading to a wide variety of responses; e.g. differentiation, proliferation, migration and invasion, angiogenesis, survival. Inhibition of PDGFA, Alpha platelet-derived growth factor receptor, PDGF-R-alpha, CD140a antigen) receptor kinase may be useful in the treatment of several tumors.

GenBank M21574

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Molecular Weight (g/mol): 63.5 kDa
• Quantity: 250 μg
• Species: Human
• Recombinant: Recombinant
• Protein Tag: N-terminal 6His
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Protein Form: Truncated
• Purity or Quality Grade: ≥62%
• Protein: PDGFRα (hu,550-end,V561D) active