PeproTech PDGF-BB Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17834893	PeproTech 500-P47BT-25UG	25 μg
17815813	PeproTech 500-P47BT-50UG	50 μg
17825003	Invitrogen 500-P47BT-1MG	2 x 500μg
30054672	PeproTech 500-P47BT-500UG	500 μg

Description

AA Sequence of recombinant protein: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB. Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PDGF-BB (500-P47) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P47BT-1MG will be provided as 2 x 500 μg

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Specifications

• Antigen: PDGF-BB
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P01127
• Gene ID (Entrez): 5155
• Immunogen: E.coli-derived, 24.3 kDa Recombinant Human PDGF-BB.
• Purification Method: Antigen affinity chromatography
• Gene: PDGFB

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: becaplermin; c-sis; FLJ12858; H-PDGF-BB; IBGC5; PDGF; pdgf protein; PDGF subunit B; PDGF, B chain; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet derived growth factor subunit B; platelet derived growth factor, B polypeptide; platelet-derived growth factor 2; platelet-derived growth factor B; platelet-derived growth factor B chain; platelet-derived growth factor beta; platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis); proto-oncogene c-Sis; Sis; SSV
• Gene Symbols: PDGFB
• Primary or Secondary: Primary
• Content And Storage: -20°C