Invitrogen™ PDCD4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA528150

Product Code. 13462237

Description

Recommended positive controls: A431, HeLa, Mouse heart. Predicted reactivity: Mouse (97%), Rat (96%), Chicken (87%), Rhesus Monkey (98%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

Specifications

• Antigen: PDCD4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PDCD4
• Gene Alias: D19Ucla1; death up-regulated gene protein; death-upregulated; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; Pdcd4; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a; protein MA-3; RP11-348N5.4; Tis; topoisomerase-inhibitor suppressed protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18569, 27250
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q53EL6, Q61823
• Gene Symbols: PDCD4
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PDCD4. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG