Invitrogen™ PAX5 Monoclonal Antibody (SP34)

Rabbit Monoclonal Antibody

Product Code	Brand	Quantity
13491117	Invitrogen™ MA516389	500 μL

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

Specifications

• Antigen: PAX5
• Concentration: 0.119 mg/mL
• Applications: ChIP Assay, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: Q02548
• Gene ID (Entrez): 500453, 5079
• Immunogen: Synthetic peptide from C-terminus of human Pax-5 protein.
• Product Type: Antibody

• Clone: SP34
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human, Rat
• Gene: PAX5
• Gene Alias: ALL3; B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; EBB-1; KLP; paired box 5; paired box gene 5; paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; paired domain gene 5; Pax5; Pax-5; Pax5 variant 1; Pax5 variant 2; RGD1565236; transcription factor PAX 5
• Gene Symbols: PAX5
• Primary or Secondary: Primary
• Purification Method: Protein A/G