IBA Lifesciences pASK-IBA E. coli vector

Product Code.	Vector	unitSize
12826486	pASK-IBA4C	5µg
12806486	pASK-IBA2C	5µg
12836486	pASK-IBA5C	5µg
12846486	pASK-IBA6C	5µg
12856486	pASK-IBA7C	5µg
12816486	pASK-IBA3C	5µg

Product Code. 12826486 Supplier IBA Lifesciences Supplier No. 21323000

Beschreibung

pASK-IBA E. coli expression vectors encoding a Strep-tag™II and chloramphenicol resistance

pASK-IBA vectors are classic cloning vectors devised for periplasmic or cytoplasmic E. coli expression of recombinant proteins with the Strep-tag™II fused to the C- or N-terminus. They carry the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI) and the inducible tetracycline promoter/operator for the regulated expression of proteins. Some carry the ompA signal for periplasmic secretion of the recombinant protein.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.

Spezifikation

• Format: Suspension
• Promoter: Tet
• Storage Buffer: TE buffer
• Antibiotic Resistance Bacterial: Chloramphenicol
• Expression System: E. coli
• Protein Tag Position (to your gene): N-terminal
• Replication Origin: F1 and ColE1 origin
• Size Range: approx. 3 kb
• Special Elements: ompA signal for periplasmic secretion
• Protein Tag: Strep-tag II
• Cloning Method: Direct Cloning
• Concentration: 250 ng/μL
• Vector: pASK-IBA4C