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PARP1 (cleaved Asp214), clone: HLNC4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6668-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15297927

  • £157.48 / 100µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH.Applications Reported: This HLNC4 antibody has been reported for use in immunoblotting (WB).Applications Tested: This HLNC4 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PARP1
HLNC4
Unconjugated
Purified
Murine
100μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
poly ADP-ribose polymerase
IgG1
RUO
Primary
Human
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