Pan Cytokeratin, eFluor 615, clone: AE1/AE3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 42-9003-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54', 50, 50', 48, and 40kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This AE1/AE3 antibody has been tested by immunocytochemistry on methanol-fixed MCF7 cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|Pan Cytokeratin (AE1/AE3)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Q29426, Q9Z2K1, Q9DCV7, Q922U2, Q61781, Q61414, Q3TTY5, P50446, P19001, P11679, P08730, P07744, P05784, P04104, P02535, P35908, P19013, P19012, P13647, P13646, P13645, P12035, P08779, P08729, P08727, P05787, P05783, P04264, P02538, P02533, Q6EIZ1, Q6EIZ0, Q6EIY9|
|CK5, DDD, EBS2, K5, KRT5A|
|Krt1, KRT10, Krt13, KRT14, KRT15, KRT16, KRT18, KRT19, Krt2, KRT3, KRT4, KRT5, KRT6A, Krt7, KRT8|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|3848, 3849, 3850, 3851, 3852, 3853, 3855, 3856, 3858, 3860, 3861, 3866, 3868, 3875, 3880|
For Research Use Only.