Pan Cytokeratin (AE1/AE3) Mouse, eFluor 615, Clone: AE1/AE3, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 42-9003-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15371120

Quantity Price
1 £ 229.69 / 100µg
Estimated Shipment date
from Supplier 05-05-2017
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Description and Specification

Specification

Host Species Murine
Isotype IgG1
Primary or Secondary Primary
Concentration 0.2mg/mL
Applications Immunocytochemistry
Applications Immunohistochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Immunohistochemistry (Frozen)
Regulatory Status RUO
Clone AE1/AE3
Quantity 100μg
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Gene Alias keratin, basic, acidic
Antigen Pan Cytokeratin (AE1/AE3)
Conjugate eFluor 615
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Species Reactivity Canine, Human, Monkey, Mouse, Rabbit, Rhesus Ma

The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.

The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54’, 50, 50’, 48, and 40kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.