PAK1/2/3 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA538311

Product Code. 15585231

Description

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Specifications

• Antigen: PAK1/2/3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PAK1
• Gene Alias: Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3, PAK3, OPHN3, PAK beta,
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18479, 18481, 224105, 29431, 29432, 29433, 5058, 5062, 5063
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 1 mg/mL
• Formulation: Dulbecco′s PBS with 150mM NaCl, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O75914, Q13153, Q13177, O88643, Q61036, Q8CIN4, P35465, Q62829, Q64303
• Gene Symbols: PAK1, PAK2, PAK3
• Immunogen: A synthetic peptide derived from the internal region of human PAK1/2/3
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG