Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder
A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.
Brand: Thermo Scientific™ 26632
Code : PN
Additional Details : Weight : 0.01250kg
DescriptionThe protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Size range – seven proteins and one peptide spanning 3.4 to 100kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands – excellent accuracy for detection in with coomassie or silver stains
Reference bands – 25kDa band is stronger than the others for easy orientation
Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.
Accurate protein sizing on SDS-polyacrylamide gels and Western blots
|PageRuler Unstained Low Range Protein Ladder|
|8 peptides and proteins from 3.4 to 100kDa in reducing sample buffer|
|50 to 100 mini gel lanes at 5 to 10μL per lane|
|2 × 250mL|