Thermo Scientific™ PageRuler™ Unstained High Range Protein Ladder

A mixture of 8 unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 26637

UNSPSC: 41105337

Code: PN

Additional Details:
Additional Details: CAS Number: 56-81-5 Weight: 5.44310kg



Product Code. 12201702

Quantity Price
1 £ 111.0 / Pack of 2
EU Stock 6
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Description and Specification

Specification

Quantity 2 × 250mL
Sufficient For 50 to 100 mini gel lanes at 5 to 10μL per lane
Formulation 8 proteins from 60 to 250kDa in reducing sample buffer
Description PageRuler Unstained High Range Protein Ladder
Stained Unstained
Detection Visible
Separation Range High

The protein MW markers in this ladder resolve into clearly identifiable, sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range 8 proteins spanning 60 to 250kDa

  • Ready-to-use supplied in a loading buffer for direct loading on gels

  • Sharp bands excellent accuracy for detection with coomassie or silver stains

  • Reference band 150kDa band has greater intensity for easy orientation

  • Tagged each protein in the ladder contains an integral Strep-tag™ II Sequence

  • Quality tested each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots