Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Overview
£90.90 valid until 31/12/2016
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Brand: Thermo Scientific™

Manufacturer Part Number: 26616

X2 PageRuler(TM) Prestained Protein

UNSPSC: 41105337

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg



Product Code. 11812124

Quantity Price
1 £ 107.0 / Pack of 2
EU Stock 435
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Description and Specification

Specification

Description PageRuler Prestained Protein Ladder, 10 to 170kDa
Detection Visible
Formulation 10 proteins from 10 to 170kDa in reducing sample buffer
Quantity 2 × 250mL
Separation Range Mid
Stained Prestained
Sufficient For 50 to 100 mini gel lanes at 5 to 10μL per lane

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Highlights:

  • Size range – 10 proteins spanning 10 to 180kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Two reference bands – orange at 70kDa and green at 10kDa

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots