p73 Antibody (5B1288) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 28 publications

Product Code	Brand	Quantity
18070074	Novus Biologicals NB100-56674SS	0.025 mg
18710424	Novus Biologicals NB100-56674	0.1 mg

Description

p73 Monoclonal specifically detects p73 in Human, Mouse samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: p73
• Concentration: 1.0 mg/mL
• Applications: Western Blot, ChIP Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Tumor Suppressors
• Gene Accession No.: O15350
• Gene ID (Entrez): 7161
• Immunogen: This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).
• Primary or Secondary: Primary
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 73 kDa

• Clone: 5B1288
• Dilution: Western Blot 1-3 ug/ml, Chromatin Immunoprecipitation 1:20 - 1:1000. Use reported in scientific literature (Accardi et al), Immunohistochemistry 1:100 - 1:200. Use reported in scientific literature (PMID 19816568), Immunocytochemistry/Immunofluorescence 1:10-1:2000. Use reported in scientific literature (Sayan et al), Immunoprecipitation 1:20 - 1:1000. Use reported in scientific literature (Sayan et al), Immunohistochemistry-Paraffin 1:100 - 1:200, Chromatin Immunoprecipitation (ChIP) 1:20-1:1000
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Gene Alias: p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
• Gene Symbols: TP73
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. NB100-56674 has been shown to recognize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.

For Research Use Only