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Invitrogen™ Multispecies p70 S6 Kinase (Total/Phospho) InstantOne™ ELISA Kit

Product Code. 15540787
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96 Tests
Unit Size:
96 tests
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15540787 96 Tests 96 tests
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Produktkod. 15540787 Leverantör Invitrogen™ Leverantörsnummer 858605311

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Beskrivning

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected.The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
TRUSTED_SUSTAINABILITY

Specifikationer

Accession Number P23443,Q8BSK8
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Mouse, Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6198, 72508
Gene Symbol PS6K,S6K,S6K-beta-1,S6K1,STK14A,p70 S6KA,p70(S6K)-alpha,p70-S6K,p70-alpha
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym RPS6KB1, Ribosomal protein S6 kinase beta-1, S6K1
Target p70 S6 Kinase
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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