Invitrogen™ p27 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA552634

Product Code. 15780581

Description

Immunogen sequence: ITFELPDNAK QCFYEDIAQG TKCTLEFQVI TGGHYDVDCR LEDPDGKVLY KEMKKQYDSF TFTASKNGTY KFCFSNEFST F Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 99%.

p27, also known as p27Kip1, is a crucial cyclin-dependent kinase inhibitor (CKI) involved in the regulation of the cell cycle. The p27 protein is encoded by the CDKN1B gene located on chromosome 12 and plays a vital role in controlling cell proliferation by inhibiting cyclin-CDK complexes, particularly cyclin A-CDK2 and cyclin E-CDK2. By regulating these complexes, p27 induces cell cycle arrest at the G1 phase, preventing cells from prematurely entering the S phase. This function is critical for maintaining normal cellular processes and preventing uncontrolled cell division. p27 acts as a tumor suppressor, and its expression is tightly controlled by transcriptional and post-transcriptional mechanisms, including proteasome-mediated degradation. Reduced levels of p27 have been associated with numerous cancers, highlighting its role in tumorigenesis. The regulation of p27 involves signaling pathways such as the PI3K/AKT pathway, impacting its localization and stability in response to extracellular signals.

Specifications

• Antigen: p27
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TMED7
• Gene Alias: CGI-109; p24 family protein gamma-3; p24g3; p24gamma3; p27; TMED7; Transmembrane emp24 domain-containing protein 7; transmembrane emp24 protein transport domain containing 7; transmembrane p24 trafficking protein 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51014
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.10 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9Y3B3
• Gene Symbols: TMED7
• Immunogen: Recombinant protein corresponding to Human p27. Recombinant protein control fragment (Product #RP-90226).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG