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p21/CIP1/CDKN1A Antibody (WA-1), Alexa Fluor™ 700, Novus Biologicals™

Product Code. 18199114 Shop All Bio Techne Products
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Product Code. 18199114 Supplier Novus Biologicals Supplier No. NBP233084AF700

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Description

Mouse Monoclonal Antibody

p21/CIP1/CDKN1A Monoclonal antibody specifically detects p21/CIP1/CDKN1A in Human, Mouse, Chimpanzee, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).
TRUSTED_SUSTAINABILITY

Specifications

Antigen p21/CIP1/CDKN1A
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone WA-1
Conjugate Alexa Fluor 700
Dilution Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Gene Alias CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
Gene Symbols CDKN1A
Host Species Mouse
Immunogen Human recombinant p21/CIP1/CDKN1A protein (Uniprot: P38936 )
Molecular Weight of Antigen 21 kDa
Purification Method Protein G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 1026
Test Specificity This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, monoclonal antibody WA-1 is useful either as a solid phase or for detection of p21 protein.
Target Species Human, Mouse, Chimpanzee, Monkey
Content And Storage Store at 4C in the dark.
Form Purified
Isotype IgG1 κ
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