Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (DFI formulation) (Dehydrated)

Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with this chromogenic medium.

Brand:  Thermo Scientific™ CM1055B

Product Code. 10076102

Description

Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (Dehydrated). A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii. The enzyme a-glucosidase, present in Chronobacter sakazakii, hydrolyses the substrate producing blue-green colonies on this pale yellow medium.

Cronobacter sakazakii is a Gram-negative rod-shaped bacterium that rarely causes disease in healthy adults. It has been implicated in outbreaks of disease in premature infants (neonates). Research suggests that neonates, or those infants who have other medical conditions, are more susceptible to this infection. Most reported cases of infection are severe, including sepsis (bacteria in the blood, meningitis, or necrotizing enterocolitis (severe intestinal infection).

Use Brilliance Cronobacter Sakazakii Agar for differentiation and enumeration of Cronobacter sakazakii from infant formula and other food samples.

• Selective: due to the presence of desoxycholate, the medium is selective against most Gram-positive organisms.
• Easy-to-read: Cronobacter sakazakii produces blue-green colonies on this medium.

Cronobacter sakazakii is an opportunistic pathogen which has been isolated at low levels from powdered infant formulas. The organisms’ high tolerance to desiccation provides a competitive advantage for Cronobacter sakazakii in dry environments, as found in milk powder factories, and thereby increases the risk of post-pasteurization contamination of the finished product¹.

The current FDA method¹ for the detection of Cronobacter sakazakii is based on yellow pigment production and originated from the pioneering work of Muytjens et al.². Samples are incubated in water overnight then enriched in EE Broth (CM0317), followed by plating on VBRGA (CM0485) to isolate Enterobacteriaceae. Five colonies are selected and streaked on Tryptone Soya Agar (CM0131), incubated for up to three days and observed for yellow colonies, typical of Cronobacter sakazakii. However, this method does not select for Chronobacter sakazakii and the combined use of EE broth and VRBGA could allow other Enterobacteria to outgrow Chronobacter sakazakii and give false negative results. It is not possible to select for Chronobacter sakazakii colonies from VRBGA plates on the basis of colony morphology as they will appear the same as other Enterobacteria.

Specifications

• Description: Brilliance E. coli/Coliform Selective Medium
• Product Type: Agar
• Yield: For 11.6L medium

• Form: Powder
• Quantity: 500 g

For Laboratory Use Only