OPTIZYME™ XhoI, Fisher BioReagents™


Overview
Brand: Fisher Bioreagents

Manufacturer Part Number: BP8010-1

Optizyme Xho I restriction enzyme

UNSPSC: 12161500

Code: 32

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11914852

Quantity Price
1 £ 26.0 / Each
Estimated Shipment date
from Supplier 03-01-2017
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Description and Specification

Specification

Product Name XhoI
Format Liquid
Concentration 10U/μL
Cut Site C.TCGAG
Incubator Temperature 37°C
pH 7.4
Includes 10X OPTIZYME™ Buffer 5
Quantity 3000U
Units 3,000U
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Xhol
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol

5'....C^T C G A G...3'
3'....G A G C T^C...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 50 - 100%
  • Buffer 4: 20 - 50%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XhoI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of XhoI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note:
  • Supercoiled plasmids may require up to 5-fold more XhoI for complete digestion than linear DNA.
  • Compatible Ends: BauI, Eco88I, SalI, SmoI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - cleavage impaired
  • CTCGAG

(Cleavage impaired)
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect

Documentation