OPTIZYME™ XbaI, Fisher BioReagents™


Overview
Brand: Fisher Bioreagents

Manufacturer Part Number: BP8008-1

Optizyme Xba I restriction enzyme

UNSPSC: 12161500

Code: 32

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11984842

Quantity Price
1 £ 26.0 / Each
EU Stock 1
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Description and Specification

Specification

Product Name XbaI
Format Liquid
Concentration 10U/μL
Cut Site T.CTAGA
Incubator Temperature 37°C
pH 7.4
Includes 10X OPTIZYME™ Buffer 4
Quantity 2000U
Units 2,000U
For Use With (Application) Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme
Storage Buffer 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol

5'...T^C T A G A...3'
3'...A G A T C^T...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 7mM 2-mercaptoethanol, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XbaI.
Digestion of Agarose-embedded DNA:
A minimum 5 units of XbaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Notes:
  • XbaI is blocked by overlapping dam methylation.
  • To avoid dam methylation, use a dam-, dcm- strain.
Compatible Ends: BcuI, Eco130I, NheI, XmaJI
Methylation Effects:
Dam: May overlap - blocked
  • 5'...TCTAGm6A TC...3'
  • 3'...AGATC Tm6AG...5'
  • (Cleavage is blocked)
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect

Documentation