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OPTIZYME™ PfoI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8077-1
Code : 32
Additional Details : Weight : 0.01000kg
Description
5'...T^C C N G G A...3'
3'...A G G N C C^T...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
- Incubate at 37°C
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 50 - 100%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
- PfoI cleavage is impaired by overlapping dam methylation and blocked by overlapping dcm methylation. To avoid dam or dcm methylation, use a dam-, dcm- strain.
- 5'...TCCNGGm6A TC...3'
- 5'...TCm5CA GGA...3'
- 5'...TCm5C GGGA...3'
Specifications
10 U/μL | |
37°C | |
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Pfo I | |
Keep container tightly closed | |
T.CCNGGA | |
Liquid |
>50% Glycerin, 25 to 50% Water | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
200 U | |
PfoI |