OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

Product Code. 11974812

Brand: Fisher BioReagents BP80581

Description

5'...A T G C A^T...3'
3'...T^A C G T A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 – 20%
• Buffer 2: 50 - 100%
• Buffer 3: 20 - 50%
• Buffer 4: 50 - 100%
• Buffer 5: 100%

Storage Buffer:

• 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NsiI.

Compatible Ends: Alw21I, PstI, SdaI, SduI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - effect not determined

Specifications

• Concentration: 10 U/μL
• Components: 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
• pH: 7.4
• For Use With (Application): >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Content And Storage: Keep container tightly closed
• Quantity: 1000 U
• Cut Site: ATGCA.T
• Product Type: NsiI
• Form: Liquid

Safety and Handling

SDS