OPTIZYME™ NcoI, Fisher BioReagents™

Product Code. 11994772

Brand: Fisher BioReagents BP80171

Description

5'...C^C A T G G...3'
3'...G G T A C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 20 - 50%
• Buffer 2: 20 - 50%
• Buffer 3: 20 - 50%
• Buffer 4: 100%
• Buffer 5: 50 - 100%

Storage Buffer:

• 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with NcoI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of NcoI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note: Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Compatible Ends: AflIII, PscI, BtgI, Eco130I, FatI, PagI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

• Concentration: 10 U/μL
• Components: 25 to 50% Water, >50% Glycerin
• pH: 7.4
• For Use With (Application): >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nco I
• Storage Buffer: 10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Content And Storage: Keep container tightly closed
• Quantity: 500 U
• Cut Site: C.CATGG
• Product Type: NcoI
• Form: Liquid

Safety and Handling

SDS