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OPTIZYME™ HindIII, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8006-5

Code : 32

Additional Details : Weight : 0.01000kg

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Product Code. 11954842

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

5'...A^A G C T T...3'
3'...T T C G A^A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and recut after a 50-fold over-digestion with HindIII.
Digestion of Agarose-embedded DNA:
A minimum 5 units of HindIII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: May overlap - cleavage impaired
  • 5'...TGm6AAGCTT(N)3 TGCT...3'
  • 3'...AC TTCGAA(N)3m6ACGA...5'
  • (Cleavage is impaired)
  • Specifications

    Specifications

    HindIII
    10U/μL
    25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
    15,000U
    10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Liquid
    A.AGCTT
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
    Keep container tightly closed
    Videos
    SDS Documentation