OPTIZYME™ HindIII, Fisher BioReagents™
Brand: Fisher Bioreagents BP8006-5
Code : 32
Additional Details : Weight : 0.01000kg
5'...A^A G C T T...3'
3'...T T C G A^A...5'
Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 0 - 20%
- Buffer 4: 50 - 100%
- Buffer 5: 100%
10mM Tris-HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and recut after a 50-fold over-digestion with HindIII.
Digestion of Agarose-embedded DNA:
A minimum 5 units of HindIII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: May overlap - cleavage impaired
- 5'...TGm6AAGCTT(N)3 TGCT...3'
|25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride|
|10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol|
|>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII|
|Keep container tightly closed|