OCT3/4 Rat anti-Human, Mouse, PE, Clone: EM92, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5841-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15268849

Quantity Price
1 £ 91.11 / 25µg
Estimated Shipment date
from Supplier 05-04-2017
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Description and Specification

Specification

Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Conjugate PE
Applications Flow Cytometry (Intracellular Staining)
Format Conjugated
Isotype IgG2a
Quantity 25μg
Antigen OCT3/4
Host Species Rat
Gene Alias OCT3, OCT4, octamer-binding transcription factor, POU5F1
Concentration 0.2mg/mL
Species Reactivity Human, Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Regulatory Status RUO
Clone EM92

The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).