Invitrogen™ O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (HGAC85)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA1076

Product Code. 11558842

Description

MA1-076 recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins. MA1-076 has been successfully used in Western blot, immunofluorescence, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects several proteins representing immunoprecipitated O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. The MA1-076 immunogen is heat killed, pepsin treated group A streptococci. DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS.

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

Specifications

• Antigen: O-linked N-acetylglucosamine (O-GlcNAc)
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites, PBS with 0.05% sodium azide
• Gene Accession No.: O15294, Q8CGY8, P56558
• Gene Symbols: OGT
• Immunogen: Heat killed, pepsin treated group A streptococci.
• Regulatory Status: RUO
• Gene ID (Entrez): 108155, 26295, 8473
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: HGAC85
• Conjugate: Unconjugated
• Gene: OGT
• Gene Alias: GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG3