Thermo Scientific™ Nunc™ Lab-Tek™ II Chambered Coverglass

Product Code.	Wells	Material	Description	unitSize
16260671	8	Borosilicate Glass (1.5)	8-well Chambered Coverglass w/ non-removable wells	Pack of 16
16200671	1	Borosilicate Glass (1.5)	1-well Chambered Coverglass w/ non-removable well	Pack of 16
10040641	1	Borosilicate Glass (1.5)	1-well Chambered Coverglass w/ non-removable well	Pack of 16
13003053	1	Borosilicate Glass (1.5)	1-well Chambered Coverglass w/ non-removable well	Pack of 96
10654602	2	Borosilicate Glass (1.5)	2-well Chambered Coverglass w/ non-removable wells	Pack of 16
13013053	2	Borosilicate Glass (1.5)	2-well Chambered Coverglass w/ non-removable wells	Pack of 96
16220671	2	Borosilicate Glass (1.5)	2-well Chambered Coverglass w/ non-removable wells	Pack of 16
16240671	4	Borosilicate Glass (1.5)	4-well Chambered Coverglass w/ non-removable wells	Pack of 16
11397714	4	Borosilicate Glass (1.5)	4-well Chambered Coverglass w/ non-removable wells	Pack of 96
10052071	4	Borosilicate Glass (1.5)	4-well Chambered Coverglass w/ non-removable wells	Pack of 16
11317724	8	Borosilicate Glass (1.5)	8-well Chambered Coverglass w/ non-removable wells	Pack of 96
10758192	8	Borosilicate Glass (1.5)	8-well Chambered Coverglass w/ non-removable wells	Pack of 16

Product Code. 16260671 Supplier Thermo Scientific™ Supplier No. 155409

Description

Culture and view living cells on a single device. This coverglass has optimum optical characteristics for high-magnification microscopy and confocal image analysis.

Culture, examine, and analyze living cells on a single device. Thermo Scientific™ Nunc™ Lab-Tek™ II Chambered Coverglass with a No. 1.5 borosilicate glass bottom offers the ideal optical characteristics required for high-magnification microscopy and confocal image analysis. Available in 1-, 2-, 4-, and 8-well formats. The upper chamber structure cannot be removed from the coverglass.

• Non-removable polystyrene chambered media chamber securely mounted to extra-thin (No. 1.5) borosilicate coverglass for optimum high-power inverted microscopic viewing
• Gas exchange achieved with minimal evaporation through tabbed polystyrene cover
• For culturing and viewing living cells
• Sterile

Ordering Information:

Shipped sterile in convenient stackable incubation trays.

Specifications

• Wells: 8
• Culture Area: 0.7 cm²
• Lid Material: Polystyrene
• Description: 8-well Chambered Coverglass w/ non-removable wells
• Surface Treatment: Cell Culture
• Material: Borosilicate Glass (1.5)
• Volume (Metric) Working: 0.2 to 0.5 mL
• Thickness (Metric): 0.16 to 0.19 mm
• Includes: Clear polystyrene lid with handles on the top and side for aseptic manipulations
• No. per Pack: 16
• Dimensions (LxW): 55 x 24 mm
• Sterility: Sterile
• Closure Color: Clear

Frequently Asked Questions (FAQs)

What can cause poor resolution when observing cells under a microscope?

Please review the following possible causes and solutions:
- The microscope could be dirty from fingerprints or oils. We recommend cleaning your microscope prior to use and always after oil immersion. When using oil immersion, it is important to use the specific brand of oil designed and recommended for that particular microscope.
- An incorrect type of coverslip or coverglass can cause poor resolution. We recommend Nunc Lab-Tek Chambered Coverglass (made from 1.0 borosilicate glass) and Nunc Lab-Tek II Chambered Coverglass (made from 1.5 borosilicate glass) for the best resolution. Plastic coverslips may affect resolution at high magnification due to refractive index differences between the glass and plastic.
- An incorrect thickness for the coverglass can cause poor resolution. Please consult your microscope's manual for the best information.
- The coverslipping procedure may affect resolution. A clear seal is required, free of debris and particles. When performing oil immersion, bubbles may result in poor resolution under high magnification.

How does cell culturing on Nunc Lab-Tek Chambered Coverglass compare with Nunc Lab-Tek II Chambered Coverglass?

Lab-Tek and Lab-Tek II chambered coverglass products can be plated with cells, incubated, and handled in the same way. The two products differ in the thickness of the glass and the chamber size. Lab-Tek coverglass uses Borosilicate coverglass No. 1 that is 0.13 - 0.16 mm thick and Lab-Tek II coverglass uses Borosilicate coverglass No. 1.5 thath is 0.16 - 0.19 mm thick. Lab-Tek II coverglass chambers hold 10 - 50% more media than Lab-Tek coverglass chambers, depending on the format. Studies have shown that Lab-Tek II chambered coverglass works well with fastidious cells such as neuronal, as well as poorly adherent cell lines.

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

What are the different options available in Lab-Tek II chamber slides?

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

What are the different options available in Lab-Tek chamber slides?

Lab-Tek chambered slide systems are based either on a standard soda lime glass microscope slide, or a culture-treated Permanox plastic slide. The latter may serve as a better culture surface for certain types of cells that don't grow well on glass, but may be of limited use for certain types of fluorescence microscopy due to the endogenous autofluorescence of plastic.

What is the cell culture treatment performed on glass Lab-Tek slides?

The standard glass Lab-Tek slide cell culture treatment consists of a proprietary, multi-stage high-purity water washing process. Unlike our plastic products for adherent cell culture (see Nunclon Delta), there is no need for further modification of the surface because glass is already hydrophilic and therefore already suitable for culture of many adherent cell lines.

What are the differences between Lab-Tek and Lab-Tek II chamber slide systems?

The main difference is the way the upper chamber structure is attached to the slide. Lab-Tek slides use a silicone gasket that can be peeled away once the chambers are removed after culture. Lab-Tek II slides use a hydrophobic acrylic adhesive that remains on the slide after chamber removal but (due to its very low profile) will not interfere with coverslips. The chamber geometry is also slightly different: on Lab-Tek slides the walls lean inward slightly to limit media evaporation, whereas on Lab-Tek II slides they are completely vertical.

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