Thermo Scientific™ Nunc™ Lab-Tek™ II CC2™ Chamber Slide System

Product Code.	Wells	Material	Description	unitSize
16290661	8	CC^2 Glass (mimics polylysine)	8-well Chamber Slide w/ removable wells	Pack of 16
10164271	1	CC^2 Glass (mimics polylysine)	1-well Chamber Slide w/ removable well	Pack of 16
13073043	1	CC^2 Glass (mimics polylysine)	1-well Chamber Slide w/ removable well	Pack of 96
16270661	1	CC^2 Glass (mimics polylysine)	1-well Chamber Slide w/ removable well	Pack of 16
13083043	2	CC^2 Glass (mimics polylysine)	2-well Chamber Slide w/ removable wells	Pack of 16
11317714	2	CC^2 Glass (mimics polylysine)	2-well Chamber Slide w/ removable wells	Pack of 96
16210651	2	CC^2 Glass (mimics polylysine)	2-well Chamber Slide w/ removable wells	Pack of 16
16280661	4	CC^2 Glass (mimics polylysine)	4-well Chamber Slide w/ removable wells	Pack of 16
10092371	4	CC^2 Glass (mimics polylysine)	4-well Chamber Slide w/ removable wells	Pack of 16
11327714	4	CC^2 Glass (mimics polylysine)	4-well Chamber Slide w/ removable wells	Pack of 96
13093043	8	CC^2 Glass (mimics polylysine)	8-well Chamber Slide w/ removable wells	Pack of 96
10564751	8	CC^2 Glass (mimics polylysine)	8-well Chamber Slide w/ removable wells	Pack of 16

Product Code. 16290661 missing translation for 'mfr' Thermo Scientific™ missing translation for 'supplierNo' 154941

Description

Enable cell growth directly on a glass microscope slide with the Thermo Scientific™ Nunc™ Lab-Tek™ II CC2™ Chamber Slide System.

Enable cell growth (e.g. neurons) directly on an aminated glass slide with the Thermo Scientific™ Nunc™ Lab-Tek™ II CC²™ Chamber Slide System, which features a chemically modified CC² growth surface that mimics the characters of polylysine coating. Available in 1-, 2-, 4-, and 8-well formats with removable media chambers.

• Remains stable and consistent for over one year without refrigeration
• Tabbed cover allows gas exchange while minimizing evaporation
• Sterile
• Removal tool separates chamber from glass
• Provides binding sites optimal for fastidious cells
• Light blue frosted writing area

Includes: Removal tool

Specifications

• Wells: 8
• Culture Area: 0.7 cm²
• Lid Material: Polystyrene
• Description: 8-well Chamber Slide w/ removable wells
• Surface Treatment: Cell Culture
• Material: CC² Glass (mimics polylysine)
• Volume (Metric) Working: 0.2 to 0.5 mL
• Includes: Clear polystyrene lid with handles on the top and side for aseptic manipulations
• No. per Pack: 16
• Dimensions (LxW): 74 x 25 mm
• Sterility: Sterile
• Closure Color: Clear

Frequently Asked Questions (FAQs)

What cell density should I use for Lab-Tek Chambered Slides and Lab-Tek II Chambered Slides?

Unfortunately, we do not have a recommended cell density for our LabTek Chamber Slides. However, you can refer to the following link, which provides recommended cell densities for our other cell culture products:
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

What are the different options available in Lab-Tek II chamber slides?

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

What are the different options available in Lab-Tek chamber slides?

Lab-Tek chambered slide systems are based either on a standard soda lime glass microscope slide, or a culture-treated Permanox plastic slide. The latter may serve as a better culture surface for certain types of cells that don't grow well on glass, but may be of limited use for certain types of fluorescence microscopy due to the endogenous autofluorescence of plastic.

What is the cell culture treatment performed on glass Lab-Tek slides?

The standard glass Lab-Tek slide cell culture treatment consists of a proprietary, multi-stage high-purity water washing process. Unlike our plastic products for adherent cell culture (see Nunclon Delta), there is no need for further modification of the surface because glass is already hydrophilic and therefore already suitable for culture of many adherent cell lines.

What are the differences between Lab-Tek and Lab-Tek II chamber slide systems?

The main difference is the way the upper chamber structure is attached to the slide. Lab-Tek slides use a silicone gasket that can be peeled away once the chambers are removed after culture. Lab-Tek II slides use a hydrophobic acrylic adhesive that remains on the slide after chamber removal but (due to its very low profile) will not interfere with coverslips. The chamber geometry is also slightly different: on Lab-Tek slides the walls lean inward slightly to limit media evaporation, whereas on Lab-Tek II slides they are completely vertical.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.