Learn More
Thermo Scientific™ Nunc™ Cell-Culture Treated Multidishes
Description
Thermo Scientific Nunc Cell-Culture Treated Multidishes receive the Nunclon Delta surface treatment. This proprietary surface modification promotes maximum adhesion for a broad range of cell types and ensures optimum performance.
Nunclon Delta surface treatment ensures optimum performance for cell adhesion.
- Flat well bottom and stackable
- Raised well rims lower risk of cross contamination
- Sterilized by gamma irradiation and certified to be non-pyrogenic
- Excellent optical quality
- Alphanumerical codes for well identification
Round well styles
- Vented lids for gas exchange
- Recessed areas around wells act as a water reservoir to reduce evaporation from wells
- Lids with notched corners for easy orientation
Rectangular well styles
- Large culture area
- Accept standard glass cover slips: 24 x 60 mm for 4-well plate and 22 x 22 mm for 8-well plate
Additional information
24-well plates are 17 mm deep; 4-well plate is 10 mm deep
Specifications
Specifications
| Certifications/Compliance | With certificate of traceability and quality (one certificate per case). |
| Culture Area | 3.5 sq. cm |
| Packaging | Individually Packed |
| Product Type | Cell Culture Dish |
| Material | Polystyrene |
| Volume (Metric) Well | 2 mL (Well) |
| No. of Wells | 12 |
| Volume (Metric) Working | 2 mL |
| Sterility | Sterile |
| Surface Treatment | Nunclon Delta |
| Show More |
Frequently Asked Questions (FAQs)
Our Nunclon Delta surface for adherent cells is a standard tissue culture (TC) surface modification that makes the polystyrene surface more hydrophilic. This facilitates maximum adhesion for a broad range of cell types.
The Nunclon Delta surface is Thermo Scientific Nunclon Delta-certified, which is a Thermo Fisher proprietary certification that has a quality standard to help ensure consistency. Every lot of Thermo Scientific Nunclon Delta-certified cell cultureware is tested with Gibco media for:
- Less than 10% growth variation in L929, Hel299 and primary chick embryo cells
- Less than 15% variation colony formation of V79-4 cells
More information and all applicable products can be found in the "Product selection guide for every step of your cell culture workflow" brochure .
This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.
Nunclon Delta refers to our performance-certified surface for culture of adherent cells, available in a variety of common formats (flasks, plates, multiwell dishes). It is tested with at least four cell lines (varies by product and manufacturing location) commonly used in research, and it should work well for most robust cell lines. It does not involve addition of coating reagent, but rather is manufactured by exposure of the molded products to a proprietary energy source, which renders the surface more hydrophilic by means of increasing the concentration of oxygen-containing (e.g. hydroxyl) molecular groups. It is by this hydrophilic property that anchorage-dependent cells are able to attach to the surface and become confluent.
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.