Thermo Scientific™ Nunc™ 96-Well Filter Plates

Product Code.	Product Type	unitSize
10134302	Fritted 96 Well DeepWell	Pack of 50
10327382	Fritted Deep Well Plate w/ Glass Fiber (Binding)	Pack of 50
10757404	Unfritted Deep Well Plate	Pack of 50

Product Code. 10134302 Supplier Thermo Scientific™ Supplier No. 278011

Description

Efficiently perform plasmid DNA filtration, PCR filtration and vacuum filtration with these Thermo Scientific™ Nunc™ 96-well filter plates.

Efficiently perform purification of high quality plasmid DNA or PCR products with the Thermo Scientific™ Nunc™ 96 Deep Well Filter Plates. Made of chemical resistant polypropylene material with superior mechanical strength, these plates conform to standard 96 well footprint compatible with high throughput sample processing.

Key Features:

• Designed specifically for high throughput sample processing
• Suitable for vacuum, filtration, centrifugatioin or positive pressure techniques
• Polyethylene terapthalate (PET) frit in fritted plate has 20μm pore size
• Shared wall configuration gives reduced plate height, wider wells for faster pocessing, higher yields and less clogging
• Purified DNA is suitable for downstream applications such as sequencing and restriction digestion
• Glass Fiber plate has PET frit and two layers of glass fiber membrane for binding DNA
• Customized bar coding is available

Recommended for:

Protein purification, lysate clarification, general filtration

Specifications

• Format: Standard
• Lid: Without Lid
• Membrane: Glass Fiber
• Array: 8 x 12
• Pore Size: 20 μm
• Color: Natural
• Product Type: Fritted 96 Well DeepWell
• Material: Polypropylene
• No. of Wells: 96
• Volume (Metric) Working: 1 mL
• Sterility: Non-sterile
• Surface Treatment: Non-treated
• Well Shape: Filter
• No. per Case: 50
• No. per Pack: 5
• Quantity: 50/Cs.

Frequently Asked Questions (FAQs)

In the protocol that accompanies Nunc 96 Deep Well Filter Plates, the vacuum pressure is measured in inches of mercury (in. HG). How do I convert that to millibar (mbar)?

29.936 in. HG equals 1.013 bars
So -5 to -10 in. Hg equals -170 to -340 mbar and -15 to -20 in. Hg equals -500 to -680 mbar

What vacuum device can I use with Nunc 96 Deep Well Filter Plates?

Any vacuum manifold (for 96-well microplates) with an inside depth of 4 cm or more should be fine.

Are the Nunc 96 Deep Well Filter Plates chemically resistant?

Yes. The polypropylene plate material naturally resists chemicals and organic solvents, as does the polyethylene terephthalate (PET) frit and glass fiber filter. For very strong chemicals, prolonged exposure times, and/or higher temperatures, we recommend that you test the plates with the chemical or solution in question.

Do I have to exactly follow the recommended protocol with Nunc 96 Deep Well Filter Plates?

The protocol is designed to be flexible, depending on customer needs and instrumentation. For example, certain steps can be performed with vacuum filtration, centrifugation, or positive pressure, with similar results. We do recommend that customers use our formulas for buffers and solutions, since they were optimized to produce superior results.

Which Nunc products can I use for high-throughput, plate-based DNA plasmid minipreps?

We recommend using the following Nunc products:

- 96-Well Polypropylene DeepWell Storage Plate, 2 mL (Cat. No. 278743) (sterile growth plate)
- Microplate Lid for 96 microwell plate (Cat. No. 264122) (to cover plate during growth)
- Polyolefin Acrylate Sealing Tape (Cat. No. 232701) (to cover plate during mixing)
- Fritted 96-Well Deep Well Filter Plate (Cat. No. 278011) (for lysate clarification)
- Fritted 96-Well Deep Well Filter Plate with Glass Fiber Filter (Cat. No. 278010) (for DNA binding)
- 96-Well Polypropylene DeepWell Storage Plate, 1.3 mL (Cat. No. 260252) (reception plate)

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.