Macherey-Nagel™ NucleoSpin™ totalRNA FFPE XS, Micro kit for RNA from FFPE

Product Code. 11922502

Brand: Macherey-Nagel™ 740969.10

Description

XS spin kit for isolation of small and large RNA from formalin-fixed, paraffin-embedded samples.

NucleoSpin FFPE RNA, no restriction for total RNA isolation - even from FFPE samples.

Enhance your RT-PCR* performance. Improved template quality - get less fragmented, well decrosslinked, DNA-free RNA.

Decrosslinking buffer and rDNase provided with the kit.Improved sensitivity - highly concentrated RNA by elution in only 5μL to 30μL.Use of the XS (mini elution volume) column design.

Convenient paraffin removal

Simply melt paraffin with the Paraffin Dissolver (patent pending) provided with the kit.Without toxic organic solvent - without time-consuming mixing steps - without long incubation time.

Save time

Minimum number of working steps.Fast procedure preserves RNA quality.

Suitable for a large variety of starting materials, like clinical samples

E.g., colon carcinoma (hematoxylin stained/unstained), colon mucosa, adenocarcinoma of colon transversum, liver (rat/human), liver carcinoma (hematoxylin stained), lymph node, spleen (rat/human); pancreas (rat/human; diabetic/nondiabetic), placenta.

• Silica membrane technology
• Format: mini spin columns - XS design for increased sensitivity
• Sample material: up to 7 sections of 250mm², or 15mg
• RNA yield depends on the amount and quality of the sample
• Typical ration A₂₆₀/A₂₈₀: 1.9 to 2.1
• Typical RIN: 2 to 6
• Typical RNA size: 100nt to 5,00nt
• Elution volume: 5μL to 30μL
• Preparation time: 70min
• Easy paraffin removal
• Convenient handling
• Fast procedure

RNA purification from FFPE samples.

Specifications

• Format: Mini Spin Column
• Final Product Type: NucleoSpin™ Total RNA FFPE XS Column
• For Use With (Application): Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples
• No. of Reactions: 10
• Product Type: NucleoSpin™ Total RNA FFPE XS Column
• Quantity: 1 Each

Safety and Handling

Signal Word

• Danger

Hazard Category

• Aspiration toxicity Category 1
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Respiratory sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H304-May be fatal if swallowed and enters airways.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P331-Do NOT induce vomiting.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 1,4-dioxane, proteinase K, paraffin dissolver, rDNase