Macherey-Nagel™ NucleoSpin™ RNA Virus F Column

For Viral RNA from up to 1 mL serum, plasma, cell-free biological fluids, enabling the processing of larger volumes

Brand:  Macherey-Nagel™ 740958

Product Code. 12788612

Description

The NucleoSpin™ RNA Virus F kit enables the processing of larger volumes. The funnel column allows handling of up to 1mL serum, plasma and other cell-free solutions with only one loading step, thus reducing cross-contamination. Due to the small diameter of the membrane the elution volume is still between 50μL to 100μL (low-salt buffer or water), resulting in a highly concentrated sample directly suitable for any amplification process. The funnel column can be centrifuged in a 50mL polyethylene tube (included in the kit) and can be closed separately. Elution tubes are fitted directly onto the funnel column. Cross contamination is eliminated because all steps are performed in a closed system. Eight or twelve samples (depending on the centrifuge rotor used) can be processed in 30 minutes. Kit components: NucleoSpin™ funnel columns, elution tubes, 50mL collecting tubes, RNase-free water, carrier RNA, buffers.

• Silica membrane technology
• Typical recovery: >90%
• Sample material: 1mL serum, plasma, cell-free biological fluids
• Fragment size: 100bp to 30kb
• Elution volume: 50μL to 100μL
• Binding capacity: 30μg
• Preparation time: 45min/2-6 preps
• Format: funnel columns
• Support protocol for parallel isolation of viral RNA and DNA (proteinase K required)
• Applicable for HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1 For purification of viral RNA and DNA from serum, plasma, cell-free biological fluids.

Specifications

• Format: Funnel Column
• For Use With (Application): For Viral RNA from up to 1 mL serum, plasma, cell-free biological fluids
• No. of Reactions: 25
• Quantity: 1 Each

• Final Product Type: NucleoSpin™ RNA Virus F Column
• Includes: Collection Tubes, collection tubes (50mL), collection tubes (0.5 mL), buffers, carrier RNA
• Product Type: NucleoSpin™ RNA Virus F Column

Safety and Handling

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4