Macherey-Nagel™ NucleoSpin™ RNA Virus, Midi kit for viral RNA from cell-free fluids

Product Code. 12788612

Brand: Macherey-Nagel™ 740958

Description

Funnel column kit for time saving parallel isolation of viral RNA / DNA from biological fluids.

The NucleoSpin™ RNA Virus F kit enables the processing of larger volumes. The funnel column allows handling of up to 1 mL serum, plasma and other cell-free solutions with only one loading step, thus reducing cross-contamination. Due to the small diameter of the membrane the elution volume is still between 50 μL to 100 μL (low-salt buffer or water), resulting in a highly concentrated sample directly suitable for any amplification process. The funnel column can be centrifuged in a 50 mL polyethylene tube (included in the kit) and can be closed separately. Elution tubes are fitted directly onto the funnel column. Cross contamination is eliminated because all steps are performed in a closed system. Eight or twelve samples (depending on the centrifuge rotor used) can be processed in 30 minutes. Kit components: NucleoSpin™ funnel columns, elution tubes, 50 mL collecting tubes, RNase-free water, carrier RNA, buffers.

• Silica membrane technology
• Typical recovery: >90%
• Sample material: 1 mL serum, plasma, cell-free biological fluids
• Fragment size: 100bp to 30kb
• Elution volume: 50 μL to 100 μL
• Binding capacity: 30 μg
• Preparation time: 45 min./2-6 preps
• Format: funnel columns
• Support protocol for parallel isolation of viral RNA and DNA (proteinase K required)
• Applicable for HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1 For purification of viral RNA and DNA from serum, plasma, cell-free biological fluids.

Specifications

• Format: Funnel Column
• Final Product Type: NucleoSpin™ RNA Virus F Column
• For Use With (Application): For Viral RNA from up to 1 mL serum, plasma, cell-free biological fluids
• Includes: Collection Tubes, collection tubes (50mL), collection tubes (0.5 mL), buffers, carrier RNA
• No. of Reactions: 25
• Product Type: NucleoSpin™ RNA Virus F Column
• Quantity: 1 Each
• Sample Size: <1 mL

Safety and Handling

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4