Macherey-Nagel™ NucleoSpin™ RNA Plus, Mini kit for RNA purification with DNA removal column

Product Code. 15370195

Brand: Macherey-Nagel™ 740984.10

Description

Mini spin kit for ultrafast and convenient RNA isolation without rDNase digestion.

During the isolation of RNA, it is imperative to prevent the degradation of the RNA. In the NucleoSpin™ RNA Plus method, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases. The lysate is added to the gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution to the flow-through, the RNA is bound to the RNA Plus Column. Two subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H₂O.

• Includes NucleoSpin™ gDNA Removal Column: no time consuming rDNase digestion
• Convenient handling: lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer: no β-mercaptoethanol or TCEP necessary
• Downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
• Kit contents: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes, buffers

Specifications

• Bacterial Content: <10⁹ Cells
• Content And Storage: Room temperature (18-25°C)
• Buffer: Lysis Buffer
• Format: Mini Spin Column
• Isolation Technology: Silica Membrane System (Two Columns)
• Elution Volume: Elution volume: 30–120μL
• Sample Type: <1 x 10⁷ Cultured Cells, <1 x 10⁹ Bacterial Cells, <1 x 10⁸ Yeast Cells, <30 mg Human/Animal Tissue
• For Use With (Application): RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Includes: NucleoSpin™ RNA Plus Columns with Collection Tubes, NucleoSpin™ gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
• No. of Reactions: 10
• Product Type: NucleoSpin RNA Plus kit
• Quantity: 1 Kit
• RNase: Yes
• Sample Size: <1 x 107 cultured cells, <30 mg human/animal tissue, <109 bacterial cells, <108 yeast cells
• Size: >200 nt
• Test Time: 20 min./6 Reaction
• Yield: HeLa cells 5 x 10⁶: 40-60μg
  Mouse liver 20mg: 80-100μg
  Mouse kidney 20mg: 40-70μg
  Mouse spleen 5mg: 30-60μg

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : ethyleneglycol diacetate / guanidinium thiocyanate /guanidine hydrochloride, ethanol