Macherey-Nagel™ NucleoSpin™ RNA Plus

Product Code.
15300205	250 Preps

Product Code. 15300205

Brand: Macherey-Nagel™ 740984.250

Description

State-of-the-art, dual-column kit includes gDNA removal columns and RNA binding columns for efficient and quick RNA isolation from cells and tissues.

NucleoSpin™ RNA Plus

• Efficient and quick RNA isolation from cells and tissues
• Two-column system includes gDNA removal columns and RNA binding columns
• NucleoSpin™ gDNA Removal Columns – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal on one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
• Variety of sample materials – cultured cells, animal tissue, plant tissue, microorganisms
• Downstream applications include real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Color: Yellow/Blue Ring
• Content And Storage: Room temperature (18°C to 25°C)
• Format: Mini Spin Column
• Isolation Technology: Silica Membrane Technology
• Elution Volume: 30 to 120 μL
• Sample Type: Cultured Bacterial or Yeast Cells, Human or Animal Tissue
• Efficiency: Binding capacity: 200 μg
• Final Product Type: RNA (with typical RIN > 9)
• For Use With (Equipment): Centrifuge
• For Use With (Application): Isolation of RNA
• Includes: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
• No. of Reactions: 250 Preps
• Product Type: RNA Isolation Kit
• Purity: A₂₆₀/A₂₈₀: 1.9 - 2.1
• Sample Size: < 30 mg tissue; < 30 mg tissue
• Test Time: 20 min./6 preps
• Yield: HeLa cells (5 x 10⁶): 40-60 μg
  Mouse liver (20 mg): 80-100 μg
  Mouse kidney (20 mg): 40-70 μg
  Mouse spleen (5 mg): 30-60 μg