Macherey-Nagel™ NucleoSpin™ RNA, Mini kit for RNA purification

Product Code. 15220023

Brand: Macherey-Nagel™ 740955.240C

Descripción

Mini spin kit for isolation of RNA of highest integrity.

Features

• DNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin™ Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set

Applications

• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater™
• RNA from saliva samples collected with Oragene™ RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Especificaciones

• Bacterial Content: 70 μg
• Detection Method: Silica-membrane
• Format: QIAcube
• Isolation Technology: Silica Membrane
• DNase: Yes
• Final Product Type: NucleoSpin™ RNA Kit
• For Use With (Application): RNA isolation from cultured cells, tissue
• No. of Reactions: 240
• No. of Samples: 4
• Product Type: NucleoSpin™ RNA Kit
• Quantity: 1 Each
• Sample Size: <5 x 106 cultured cells, <30 mg human/animal tissue, <109 bacterial cells, <108 yeast cells
• Yield: 14μg HeLa cells, 70μg Bacterial Cells

Seguridad y manipulación

Palabra de advertencia

• Danger

Clasificación

• Flammable liquid Category 3
• Acute toxicity Category 4
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Indicaciones de peligro

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Consejos de prudencia

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Otros peligros

• MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) / guanidine hydrochloride, ethanol