Macherey-Nagel™ NucleoSpin™ RNA Columns

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

Product Code	Brand	Quantity
15880968	Macherey-Nagel™ 740955.50S	50 Columns
15870968	Macherey-Nagel™ 740955.250S	250 Columns

Description

NucleoSpin™ RNA

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
• RNA isolation from cultured cells, tissue (standard protocol):
  • RNA from < 10⁹ bacterial cells (Gram-negative, Gram-positive) or < 10⁸ yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater™
  • RNA from saliva samples collected with Oragene™ RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Color: Blue band
• Format: Mini Spin Column
• Elution Volume: 40 - 120 μL
• Efficiency: Binding capacity: 200 μg
• For Use With (Application): Isolation of RNA from cells and tissue
• Sample Size: < 30 mg tissue, 5 x 10⁶ cultured cells

• Content And Storage: rDNase at 4 °C; Other components at room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane
• Sample Type: Cultured Bacterial or Yeast Cells, Human or Animal Tissue
• For Use With (Equipment): NucleoSpin RNA Kits
• Includes: Columns only
• Test Time: 35 min/6 preps