Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification

Artikelnummer. 15880968

Marke: Macherey-Nagel™ 740955.50S

Beschreibung

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

NucleoSpin™ RNA

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
• RNA isolation from cultured cells, tissue (standard protocol):
  • RNA from < 10⁹ bacterial cells (Gram-negative, Gram-positive) or < 10⁸ yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater™
  • RNA from saliva samples collected with Oragene™ RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Spezifikation

• Color: Blue band
• Content And Storage: rDNase at 4 °C; Other components at room temperature (18°C to 25 °C)
• Format: Mini Spin Column
• Isolation Technology: Silica Membrane Technology
• Elution Volume: 40 to 120 μL
• Sample Type: Cultured Bacterial or Yeast Cells, Human or Animal Tissue
• Efficiency: Binding capacity: 200 μg
• For Use With (Equipment): NucleoSpin RNA Kits
• For Use With (Application): Isolation of RNA from cells and tissue
• Includes: Columns only
• Quantity: 50 Columns
• Sample Size: < 30 mg tissue, 5 x 10⁶ cultured cells
• Test Time: 35 min/6 preps

Sicherheit und Handhabung

Produktidentifikator

• NucleoSpin RNA

Ergänzende Informationen

• MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu